Product Name :
PACT (PKR activating protein) / PRKRA Recombinant Rabbit Monoclonal Antibody [PSH02-15]
Predicted band size :
34 kDa
Observed band size :
34 kDa
Synonyms:
DYT16 antibody HSD14 antibody Interferon inducible double stranded RNA dependent protein kinase activator A antibody interferon-inducible double stranded RNA-dependent activator antibody Interferon-inducible double stranded RNA-dependent protein kinase activator A antibody PACT antibody PKR associated protein X antibody PKR associating protein X antibody PKR-associated protein X antibody PKR-associating protein X antibody 展开
Function :
PRKRA can activate EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. Required for siRNA production by DICER1 and for subsequent siRNA-mediated post-transcriptional gene silencing. Does not seem to be required for processing of pre-miRNA to miRNA by DICER1. Promotes UBC9-p53/TP53 association and sumoylation and phosphorylation of p53/TP53 at ‘Lys-386’ at ‘Ser-392’ respectively and enhances its activity in a EIF2AK2/PKR-dependent manner.
Antibody Type:
Recombinant Rabbit monoclonal Antibody
Immunogen :
Recombinant protein within human PACT aa 1-313 (O75569).
Species reactivity:
Human, Mouse, Rat
Verify application:
WB, IHC-P, IF-Cell, IF-Tissue, FC
Coupling :
unconjugated
Clone number:
PSH02-15
RRID:
AB_3072886
Form :
Liquid
Concentration :
1ug/ul
Storage instructions:
Store at +4℃ after thawing.1-(6-Bromonaphthalen-2-yl)ethanone Chemscene Aliquot store at -20℃.181434-36-6 manufacturer Avoid repeated freeze / thaw cycles.PMID:33705529
Storage buffer solution:
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Subtype:
IgG
Purification method :
Protein A affinity purified.
ELTSA :
Molecular weight :
Predicted band size: 34 kDa
Positive control :
293T cell lysate, K-562 cell lysate, Jurkat cell lysate, HepG2 cell lysate, MCF7 cell lysate, Hela cell lysate, HL-60 cell lysate, C2C12 cell lysate, PC-12 cell lysate, mouse testis tissue lysate, mouse liver tissue lysate, rat testis tissue lysate, rat brain tissue lysate, human testis tissue, rat brain tissue, rat cerebral cortex tissue, rat hippocampus tissue, 293T, PC-12.